Human Genome Sequencing Center, Baylor College of Medicine

BAC-Fisher Loader

The BAC-Fisher server collects multiple submissions and runs at set times throughout the day. When your job is complete, you will be e-mailed the URL for downloading the results.

For more information about the BAC-Fisher, see the introduction, and the Release Notes.

The BAC-Fisher is experimental software in active development. Use your own discretion in applying the results.

For more information on individual organisms, please refer to our project page.

Choose which whole-genome pool you want to fish in:	Apis mellifera (honey bee) Bos taurus (cow) Drosophila pseudoobscura (fruit fly) Homo sapiens (human) Macaca mulatta (rhesus macaque) Mus musculus (mouse) Rattus norvegicus (rat) Strongylocentrotus purpuratus (purple sea urchin)
Enter your e-mail address here
Upload the bait sequences in Fasta format here:
Bait should be from a single gene or localized genomic region.
The bait sequences I am submitting are:	With Quality: genomic reads with corresponding Fasta quality file given below. Highly Recommended. Clipped: genomic reads with low-quality and vector sequence clipped off. Warning: do not submit unclipped reads without quality -- you are likely to get empty or false results. Gene: expressed gene sequences (EST, mRNA or cDNA). These should also be high-quality sequences, but finished sequences longer than reads are allowed.
Upload Fasta quality file for the bait sequences:
Choose the results you want to receive
The results I want to receive are:	fasta and quality files of overlapping reads assembled contigs from Phrap assembled scaffolds (contigs ordered and oriented into longer sequences with Ns for gaps)

Submission Hints

Submitted sequence files must be plaintext FASTA format or gzip'ed FASTA format.

>readname rest of line does not matter (for now)
ACGTACGT
GTCAGTCA
>readname2 rest of line ignored
TCCGTTA

Submitted quality files must be plaintext FASTA quality format or gzip'd FASTA quality format.

>readname readname must agree with sequence file
10 20 30 40 30 35 40 32
37 21 25 20 19 17 13 5
>readname2 number of quality values must agree with number of bases in sequence file
6 13 29 37 42 40 11

http://www.hgsc.bcm.tmc.edu/BAC-Fisher/BAC-Fisher-loader.cgi

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