About the Project
NCBI Trace Archive
The BCM-HGSC is sequencing three species of parasitic wasp: Nasonia vitripennis, Nasonia giraulti, and Nasonia longicornis.
Nasonia is a genus containing these three parasitic wasps whose hosts include house flies and other filth flies.
It is a genetic model for all parasitic wasps that are used in the biological control of insect pests. Because of its usable haploid genetics, Nasonia is a facile model for discovering the genes behind complex traits. Nasonia will be the second representative of the Hymenoptera to be sequenced at the BCM-HGSC.
N. vitripennis has been sequenced using a WGS strategy with a six-fold sequence coverage, whereas N. giraulti and N. longicornis are being sampled at single-fold sequence coverage, and will be aligned to the N. vitripennis assembly to identify and localize sequence markers necessary for mapping quantitative traits to individual gene loci.
Access to the Data
Version 1.0 of the assembly, Nvit_1.0 is available for download using the FTP Data link in the sidebar. The assembly is described in detail in the README in that directory. Nvit_1.0 is a preliminary genome assembly that includes large insert paired end information and assembly of repetitive sequences but has not been localized to chromosomes.
Comparative Genome Sequence Alignments
We have updated our comparative genome sequence alignments of the giraulti and longicornis genomes to the vitripennis reference genome, and produced BAM files.
This will enable easier processing of the alignments using the SAMTOOLS and GATK and other software suites, and easier visualization of the alignments using IGV (the integrated genome viewer).
Find Bam files, indexes, consensus sequences, and the commands used to generate them.
The BLAST link is located in the sidebar.
Linearized scaffold sequences
Traces are available from the NCBI Trace Archive by using the link in the sidebar, or by using NCBI MegaBLAST with a same species or cross species query.
Selected PubMed Citations
Savard, J, Tautz, D, Richards, S, Weinstock, GM, Gibbs, RA, Werren, JH, Tettelin, H, Lercher, MJ. Phylogenomic analysis reveals bees and wasps (Hymenoptera) at the base of the radiation of Holometabolous insects. Genome Res. 2006 Nov; 16(11):1334-8. [PubMed]