Drosophila Genome Project

Image source: Wikimedia Commons

The BCM-HGSC has been involved in several Drosophila genome sequence projects.

Drosophila Genetic Reference Panel

In association with Trudy Mackay (North Carolina State University) we are sequencing a number of phenotyped inbred strains of D. melanogaster using new technologies. The aim is to allow whole genome association studies, and make both the fly lines and the sequences available to the entire Drosophila community. As a start to this process, sequences will be made available here as well as in the usual public depositories.

Drosophila melanogaster genome sequence

The BCM-HGSC was a member of the Berkeley Drosophila Genome Project (BDGP), and responsible for sequencing and finishing approximately one third of the D. melanogaster reference sequence. The reference has since been published (Adams, M. D. et al. The genome sequence of Drosophila melanogaster. Science 287, 2185-95 (2000).) and further refined (Celniker, S. E. et al. Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence. Genome Biol 3, RESEARCH0079 (2002)).

Find the most recent data at flybase.

Drosophila pseudoobscura genome sequence

A draft genome sequence of the fruitfly (D. pseudoobscura) was performed by the BCM-HGSC, and published in January 2005 (Richards et al. Comparative genome sequencing of Drosophila pseudoobscura: Chromosomal, gene, and cis-element evolution. Genome Research 15:1-18).

Drosophila modENCODE Project

As part of the Drosophila modENCODE project, we are studying the comparative genomics of eight additional species of Drosophila: biarmipes, bipectinata, elegans, eugracillis, ficusphila, kikkawai, rhopaloa and takahashii.


 

Related Publications

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Bogaert E, Garde A, Gautier T, Rooney K, Duffourd Y, LeBlanc P, et al.. SRSF1 haploinsufficiency is responsible for a syndromic developmental disorder associated with intellectual disability. Am J Hum Genet. 2023 ;110(5):790-808.

Chen Q, Sasikala-Appukuttan AKirshna, Husain Z, Shrivastava A, Spain M, Sendler ED, et al.. Global Gene Expression Analysis Reveals Complex Cuticle Organization of the Tribolium Compound Eye. Genome Biol Evol. 2023 ;15(1).

Yeung K, Raja KKumar Boll, Shim Y-K, Li Y, Chen R, Mardon G. Single cell RNA sequencing of the adult Drosophila eye reveals distinct clusters and novel marker genes for all major cell types. Commun Biol. 2022 ;5(1):1370.

Chung H-L, Rump P, Lu D, Glassford MR, Mok J-W, Fatih J, et al.. De novo variants in EMC1 lead to neurodevelopmental delay and cerebellar degeneration and affect glial function in Drosophila. Hum Mol Genet. 2022 ;31(19):3231-3244.

Yap ZYie, Park YHan, Wortmann SB, Gunning AC, Ezer S, Lee S, et al.. Functional interpretation of ATAD3A variants in neuro-mitochondrial phenotypes. Genome Med. 2021 ;13(1):55.

Barish S, Barakat TStefan, Michel BC, Mashtalir N, Phillips JB, Valencia AM, et al.. BICRA, a SWI/SNF Complex Member, Is Associated with BAF-Disorder Related Phenotypes in Humans and Model Organisms. Am J Hum Genet. 2020 ;107(6):1096-1112.

Link N, Chung H, Jolly A, Withers M, Tepe B, Arenkiel BR, et al.. Mutations in ANKLE2, a ZIKA Virus Target, Disrupt an Asymmetric Cell Division Pathway in Drosophila Neuroblasts to Cause Microcephaly. Dev Cell. 2019 ;51(6):713-729.e6.

Justice AE, Karaderi T, Highland HM, Young KL, Graff M, Lu Y, et al.. Protein-coding variants implicate novel genes related to lipid homeostasis contributing to body-fat distribution. Nat Genet. 2019 ;51(3):452-469.

Wiegmann BM, Richards S. Genomes of Diptera. Curr Opin Insect Sci. 2018 ;25:116-124.