Title | Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Csuros, M, Li, B, Milosavljevic, A |
Journal | Genome Inform |
Volume | 14 |
Pagination | 186-95 |
Date Published | 2003 |
ISSN | 0919-9454 |
Keywords | Animals, Chromosomes, Artificial, Bacterial, Computational Biology, Computer Simulation, Drosophila melanogaster, Genome, Reproducibility of Results, Research Design, Sequence Alignment |
Abstract | This paper studies sequencing and mapping methods that rely solely on pooling and shotgun sequencing of clones. First, we scrutinize and improve the recently proposed Clone-Array Pooled Shotgun Sequencing (CAPSS) method, which delivers a BAC-linked assembly of a whole genome sequence. Secondly, we introduce a novel physical mapping method, called Clone-Array Pooled Shotgun Mapping (CAPS-MAP), which computes the physical ordering of BACs in a random library. Both CAPSS and CAPS-MAP construct subclone libraries from pooled genomic BAC clones. |
Alternate Journal | Genome Inform |
PubMed ID | 15706533 |
Grant List | R01 HG02583-01 / HG / NHGRI NIH HHS / United States U01 RR18464 / RR / NCRR NIH HHS / United States |
Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments.
Similar Publications
FAIR Header Reference genome: a TRUSTworthy standard. Brief Bioinform. 2024;25(3). | .
Core planar cell polarity genes and in predisposition to congenital vertebral malformations. Proc Natl Acad Sci U S A. 2024;121(18):e2310283121. | .
NODAL variants are associated with a continuum of laterality defects from simple D-transposition of the great arteries to heterotaxy. Genome Med. 2024;16(1):53. | .