| Title | High mobility group (HMG-box) genes in the honeybee fungal pathogen Ascosphaera apis. |
| Publication Type | Journal Article |
| Year of Publication | 2007 |
| Authors | Aronstein, KA, Murray, KD, de León, JH, Qin, X, Weinstock, GM |
| Journal | Mycologia |
| Volume | 99 |
| Issue | 4 |
| Pagination | 553-61 |
| Date Published | 2007 Jul-Aug |
| ISSN | 0027-5514 |
| Keywords | Amino Acid Sequence, Animals, Bees, Computational Biology, Fungal Proteins, Gene Expression Regulation, Fungal, Genes, Mating Type, Fungal, HMG-Box Domains, Molecular Sequence Data, Onygenales, Phylogeny, Polymerase Chain Reaction, Transcription Factors |
| Abstract | The genome of the honeybee fungal pathogen Ascosphaera apis (Maassen) encodes three putative high mobility group (HMG-box) transcription factors. The predicted proteins (MAT1-2, STE11 and HTF), each of which contain a single strongly conserved HMG-box, exhibit high similarity to mating type proteins and STE11-like transcription factors previously identified in other ascomycete fungi, some of them important plant and human pathogens. In this study we characterized the A. apis HMG-box containing genes and analyzed the structure of the mating type locus (MAT1-2) and its flanking regions. The MAT1-2 locus contains a single gene encoding a protein with an HMG-box. We also have determined the transcriptional patterns of all three HMG-box containing genes in both mating type idiomorphs and discuss a potential role of these transcription factors in A. apis development and reproduction. A multiplex PCR method with primers amplifying mat1-2-1 and Ste11 gene fragments is described. This new method allows for identification of a single mating type idiomorph and might become an essential tool for applied and basic research of chalkbrood disease in honeybees.
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| DOI | 10.3852/mycologia.99.4.553 |
| Alternate Journal | Mycologia |
| PubMed ID | 18065006 |