Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice.

Title	Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice.
Publication Type	Journal Article
Year of Publication	2019
Authors	Park, YH, Snook, JD, Ostrin, EJ, Kim, S, Chen, R, Frankfort, BJ
Journal	Sci Rep
Volume	9
Issue	1
Pagination	2594
Date Published	2019 Feb 22
ISSN	2045-2322
Keywords	Animals, Biomarkers, Disease Models, Animal, Female, Glaucoma, Intraocular Pressure, Male, Mice, Mice, Inbred C57BL, Retinal Ganglion Cells, Sequence Analysis, RNA, Transcriptome
Abstract	Elevated intraocular pressure (IOP) is the major risk factor for glaucoma, a sight threatening disease of retinal ganglion cells (RGCs) and their axons. Despite the central importance of IOP, details of the impact of IOP elevation on RGC gene expression remain elusive. We developed a 4-step immunopanning protocol to extract adult mouse RGCs with high fidelity and used it to isolate RGCs from wild type mice exposed to 2 weeks of IOP elevation generated by the microbead model. IOP was elevated to 2 distinct levels which were defined as Mild (IOP increase >1 mmHg and
DOI	10.1038/s41598-019-39141-1
Alternate Journal	Sci Rep
PubMed ID	30796289
PubMed Central ID	PMC6385489
Grant List	P30 EY002520 / EY / NEI NIH HHS / United States S10 OD023469 / OD / NIH HHS / United States