About DGRP

An amazing phenotypic resource: Our collaborator Trudy Mackay has a large set of > 200 inbred D. melanogaster lines with significant amounts of quantitative phenotypic data. These will form the basis of a community wide Drosophila Genetic Reference Panel (DGRP) where members of the community can perform phenotypic assays on a presequenced set of Drosophila lines for performing association studies at high power. This approach amortizes the sequencing costs over the entire Drosophila community. The figure provides data on three phenotypes, male aggressiveness, ethanol sensitivity and lifespan. The Mackay laboratory also has data on life span, alcohol sensitivity, male aggression, male copulation latency, locomotive behavior, starvation resistance, chill coma recovery, abdominal bristle number, sternal pleural bristle number, adult mRNA transcription levels and olfactory behavior. Additionally many other laboratories have promised to assay hundreds of additional phenotypes once sequence is available.

Community Involvement

Anyone can sign up for the public mailing list for DGRP-related discussion.

There is also an analysis-oriented blog where discussion can more easily be accompanied by relevant graphs, images, etc.

Data Release

As a service to the community we are releasing all sequence data for this project pre-publication and as soon as possible here - often pre-analysis on this ftp site. The data is released here under the standard genome sequence release agreements.

Users are free to use the data in scientific papers analyzing particular genes and regions if the providers of this data are properly acknowledged. Please cite the BCM-HGSC web site or publications from BCM-HGSC referring to the genome sequence. BCM HGSC plans to publish the assembly and genomic annotation of the dataset, including genome wide molecular population genetic analyses, large-scale identification of regions of evolutionary conservation and quantitative trait association studies. This is in accordance with, and with the understandings in the Fort Lauderdale meeting discussing Community Resource Projects and the resulting NHGRI policy statement.

Data will also be deposited into the trace archive and other appropriate depositories once all QC/QA tests are passed.

DGRP line numbers correspond to Bloomington Drosophila Stock Center designations for these lines: e.g., DGRP_360 is the same as RAL-360.

Berkeley Drosophila Reference Sequencing Strain ((y[1]; cn[1] bw[1] sp[1]) b) Adams, M. D. et al. The genome sequence of Drosophila melanogaster. Science 287, 2185-95 (2000). – This strain was kindly provided by Dr, Susan Celiniker at the BDGP. It is also available at the Bloomington Drosophila Stock Center.

DGRP line 375 - 12X 454-long read (500bp) data, aligned sequence in fastm format. (The long read data was generated in house by 454 in Dec 2007 and Jan 2008 prior to the release of their next generation long read platform. We thank 454 Inc. for allowing us to release this data to the public, pre-publication.)

Data for all other lines was produced by the BCM-HGSC and is available here.